Anti-TNFSF4 Neutralizing Antibody (V3S-0622-YC5690)

Figure 1 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in SDS-PAGE
Figure 2 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in SEC-HPLC
Figure 3 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in WB
Figure 4 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in ELISA

Datasheet

MSDS

COA

Summary

Description	This product is a human monoclonal antibody that reacts with TNFSF4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality	Monoclonal
Host Species	Human
Target Species	Human
Isotype	IgG

Property

Expression Species	HEK293F or CHO
Conjugation	None
Purity	>95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin	<1 EU/mg, determined by LAL method
Purification	Protein A affinity purified
Sterility	0.2 μM filtered
Formulation	PBS, pH 7.4
Preservation	No preservatives
Stabilizer	No stabilizers
Storage	Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Tested Data

SDS-PAGE

Figure 1 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC5690 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 175-270 kDa.

Figure 1 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in SDS-PAGE

SEC-HPLC

Figure 2 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in SEC-HPLC

The purity of V3S-0622-YC5690 was greater than 95% as determined by SEC-HPLC.

Figure 2 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in SEC-HPLC

Figure 3 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in WB

Western blot analysis of V3S-0622-YC5690 was performed by loading Recombinant Human CD252 Protein.

Lane 1: Non-Reducing Antigen (1 μg)
Lane 2: Reducing Antigen (1 μg)

Figure 3 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in WB

ELISA

Figure 4 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in ELISA

ELISA analysis of V3S-0622-YC5690 was performed by coating with Recombinant Human CD252 Protein.

Figure 4 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in ELISA

Application	WB; ELISA; FC; IP; FuncS; IF; Neut; ICC
Application Notes	The antibody is recommended for detection of TNFSF4 by ELISA, FC, IP, FuncS, IF, Neut, ICC assays.

ELISA	Enzyme-Linked Immunosorbent Assay Protocol
WB	Western Blot Protocol
FC	Flow Cytometry Protocol

Target	TNFSF4
Alternative Name	TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tax transcriptionally activated glycoprotein 1, 34kD, TXGP1; tumor necrosis factor ligand superfamily member 4; CD252; gp34; OX 40L; OX40L; CD134 lig
Gene ID	7292
UniProt	P23510
Research Area	Immunology

Anti-TNFSF4 Neutralizing Antibody (V3S-0622-YC5690) (CAT#: V3S-0622-YC5690)

Summary

Property

Applications

Protocols

Target

Tested Data

Anti-TNFSF4 Neutralizing Antibody (V3S-0622-YC5690) (CAT#: V3S-0622-YC5690)

Summary

Property

Applications

Protocols

Target

Tested Data

Isotype Control

Secondary Antibody

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