| Description | This mouse antibody (clone SC16.62) screened from hybridoma recognizes human DLL3 (delta like canonical Notch ligand 3). This anti-DLL3 antibody binds to the liner epitope in the EGF3 domain (aa 312-351) as determined by Flow Cytometry. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Human DLL3-fc protein (hDLL3-Fc) |
| Epitope | EGF3 domain, aa 312-351 (linear epitope) |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; FC |
| Application Notes | Antibody SC16.67 is recommended for detection of human DLL3 by ELISA. The epitope mapping was measured by Flow Cytometry. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Post Translational Modification | Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. |
