| Description | This T-cell receptor mimic (TCRm) antibody binds to human p53 tumor suppressor protein residues 65-73 (RMPEAAPPV) when presented by the MHC class I protein Human Leukocyte Antigen-A*0201 (HLA-A*0201). It can bind to the surface of HLA-A2+/p53+ tumor cell lines and engage immune effector functions including CDC, ADCP, ADCC. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | A human tetramers composed of the human HLA-A*0201, p2-microglobulin (β2m) and an immunogenic peptide derived from the human p53 protein. |
| Epitope | aa 65-73 (RMPEAAPPV) |
| Isotype | Mouse IgG2a |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; FC; CDC; ADCP; ADCC |
| Application Notes | The ability of this antibody to recognize the human p53 (residues 65-73) peptide/HLA*0201 complex on the cell surface was tested by Flow Cytometry. This antibody T1-116C can bind to the surface of HLA-A2+/p53+ tumor cell lines and engage immune effector functions including CDC, ADCP, ADCC. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | p53 TCRm |
| Alternative Name | p53 T-cell receptor mimic; TCRm |
| Research Area | Immunology; Cell Biology; Cancer Research |
| Related Disease | Cancer |
