| Description | This is an inhibitory monoclonal antibody that specifically recognizes Pfs25. It binds near the canonical site 1 epitope. This antibody has the highest affinity for recombinant Pfs25 within its respective epitope bin and showed>80% oocyst reduction in SMFA at 100 μg/mL. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Plasmodium falciparum |
| Epitope | Near the canonical site 1 epitope. |
| Affinity | KD = 4.6 nM |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib |
| Application Notes | This antibody showed>80% reduction in oocyst density at 100 μg/mL. mAb 2544 is more potent than any other identified anti-Pfs25 antibody (IC₈₀ = 16 μg/mL) and has much less potent recombinant Pfs25 binding affinity in the same low nanomolar range (KD = 4.6 nM) as multiple other mAbs. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Pfs25 |
| Alternative Name | Plasmodium falciparum transmission; Plasmodium falciparum; P. falciparum |
| Research Area | Immunology |
