Anti-LTA Neutralizing Antibody (V3S-0622-YC2110) (CAT#: V3S-0622-YC2110)

Figure 1 Anti-Human Lymphotoxin alpha Monoclonal Antibody (V3S-0622-YC2110) in ELISA

ELISA analysis of V3S-0622-YC2110 was performed by coating with Recombinant Human TNF beta Protein. Then blocked with BSA, and incubated with Anti-Human TNF beta Antibody (V3S-0622-YC2110). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human Lymphotoxin alpha Monoclonal Antibody (V3S-0622-YC2110) in WB

Western blot analysis of V3S-0622-YC2110 was performed by loading Recombinant Human TNF beta Protein (lane 1, 0.1 μg; lane 2, 0.3 μg; lane 3, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2110 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.

Figure 3 Anti-Human Lymphotoxin alpha Monoclonal Antibody (V3S-0622-YC2110) in DB

Antigen: Recombinant human TNF beta protein
Antibody incubation concentration: 2ng/μL.

The secondary antibody: HRP-conjugated goat anti-human IgG

Figure 4 Anti-Human Lymphotoxin alpha Monoclonal Antibody (V3S-0622-YC2110) in SEC-HPLC

The purity of V3S-0622-YC2110 was greater than 97% as determined by SEC-HPLC.

Figure 5 Anti-Human Lymphotoxin alpha Monoclonal Antibody (V3S-0622-YC2110) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC2110 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.