| Description | The humanized anti-biotin antibody was generated (huM33) based on the human germline framework IGHV 1-69-02 and IGKV 1-27-01 combination. The anti-biotin antibody was expressed by transient transfection of human embryonic kidney 293 (HEK293) or chinese hamster ovary (CHO) cells in suspension. The binding properties of the recombinant humanized antibody was analyzed by biolayer interferometry (BLI) technology. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Non-species dependence |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | SPR |
| Application Notes | The antibody is recommended for detection of Biotin by SPR assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Biotin |
| Alternative Name | Biotin |
Figure 1 Afucosylated Recombinant Anti-Biotin Antibody (V3S-0622-YC43) in Western Blot.
Western blot analysis of V3S-0622-YC43 was performed by loading a antibody with biotin conjugated.
V3S-0622-YC43 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 1 Afucosylated Recombinant Anti-Biotin Antibody (V3S-0622-YC43) in Western Blot.
Figure 2 Afucosylated Recombinant Anti-Biotin Antibody (V3S-0622-YC43) in ELISA.
ELISA analysis of V3S-0622-YC43 was performed by coating with an antibody with biotin conjugated. Then blocked with BSA and incubated with Afucosylated recombinant anti-Biotin Antibody (V3S-0622-YC43). The goat anti-human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Afucosylated Recombinant Anti-Biotin Antibody (V3S-0622-YC43) in ELISA.
