| Description | This product is a human monoclonal antibody against the anthrax toxin protective antigen (PA, 83 kDa before enzymatic cleavage and 63 kDa after cleavage). |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Bacillus anthracis |
| Affinity | 0.33 pM |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Neut; FuncS |
| Application Notes | The 35PA83 "6.20" IgG exhibits, with respect to the PA83 antigen, an association constant (Ka) of 2.93×105 M, a dissociation constant (Kd) of 9.70×10-6 M and an affinity constant (KD) of 3.3×10-11 M. 35PA83 has a high-affinity antibody which effectively neutralizes the lethal toxin (IC50 = 5.6 nM). The 35PA83 antbody IgG neutralizes the anthrax toxin by preventing the interaction of PA with its cell receptor. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Anthrax PA |
| Alternative Name | Anthrax toxin PA |
| Research Area | Infectious Disease |
