| Description | The humanized IgG/k versions of the murine mAb against beta-amyloid polypeptide were created. The humanized mAb was produced by transient transfections in CHO-S or HEK293F cells. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Affinity | The KD ranges, 0.182 nM-0.463 nM, were determined using 2:1 kinetics of binding of analyte (BSA-cSNK). |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB |
| Application Notes | ELISA Assay of Humanized 5E3 (hu5E3) mAb An ELISA was used to test the binding of hu5E3 against cSNK-BSA peptide. The ELISA plate was coated with antigen (Ag), 1 μg/well of cSNK-BSA. The wells were blocked with 5% skim milk then probed with serially diluted the mAbs (0.01 μg/mL to 5 μg/mL) and binding was detected with a commercial goat anti-human HRP conjugate antibody. The plate was read at 450 nm. Western Blot Analysis of Humanized 5E3 (hu5E3) mAb A 4-12% gradient SDS-PAGE gel was run with cSNK-BSA. The gel was then transferred to a nitrocellulose membrane. The next day the mAbs were diluted to 2 μg/ml to 5 μg/ml depending on the antibody, and used to probe the membrane at room temperature (RT). The membranes were then probed with anti-human IgG-HRP. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | APP |
| Alternative Name | AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma; Amyloid-beta precursor protein; Amyloid-beta A4 protein; Alzheimer disease amyloid protein; Amyloid beta (A4) precursor protein; Amyloid beta A4 protein; Amyloid precursor protein; Beta-amyloid peptide; Beta-amyloid peptide(1-40); Beta-amyloid peptide(1-42); Beta-amyloid precursor protein; Cerebral vascular amyloid peptide; Peptidase nexin-II; Protease nexin-II; Testicular tissue protein Li 2 |
| Gene ID | 351 |
| UniProt | P05067 |
| Research Area | Neuroscience |
