| Description | This product is a monoclonal antibody to angiotensin II derived from mouse. The antibody displayed substantially less cross-reactivity with angiotensin III. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Mammal |
| Immunogen | Angiotensin II coupled to keyhole limpet hemocyanin (KLH). |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | Neut; RIA; ELISA |
| Application Notes | Monoclonal antibody KAA8 has high affinity for angiotensin II (ka = 1.1×10⁹M⁻¹) and its relatively low cross-reactivity for angiotensin III (32%). The mAb would be particularly suited to measure angiotensin II with limited interference from angiotensin III. In animal models, doses of this antibody monoclonal antibody as low as 1.5 mg/kg were effective in reducing blood pressure. Maximum anti-hypertensive effects were observed with the antibody doses of 15 mg/kg. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Ang II |
| Alternative Name | Angiotensin II; KAA8 |
