| Description | The recombinant antibodies, which were derived from a single monoclonal antibody against artemisinin (AM) and artesunate (AS). The recombinant antibody provides an essential component of an economically attractive immunoassay and will be useful in other immunochemical applications for the analysis and purification of antimalarial drugs. It was sufficient for analysis of antimalarial drugs, and its utility for quality control of analysis of Artemisia spp was validated. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Non-species dependence |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA |
| Application Notes | Indirect competitive ELISA (icELISA) assay Artesunate-OVA (1 μg/mL) dissolved in 50 mM carbonate buffer was adsorbed to the wells of a 96-well immunoplate. The plate was treated with 300 μL of S-PBS for 1 h to reduce nonspecific adsorption. Aliquots (50 μL) of various concentrations of artemisinin (AM) or sample solutions dissolved in 20% MeOH were incubated with 50 μL of AM-Fab solution (5 μg/mL) in SPBS for 1 h. The plate was washed three times with TPBS, then incubated with 100 μL of a 1000× diluted HRP-labeled antimouse Fab antibody solution for 1 h. After washing the plate three times with TPBS, 100 μL of the ABTS solution were added to each well and incubated for 15 min. The absorbance at 405 nm was measured using a microplate reader. All reactions were carried out at 37 °C. It has proved that this antibody Fab fragment sensitivity of the icELISA was 0.16 μg/mL to 40 μg/mL for artemisinin (AM) and 8.0 ng/mL to 60 ng/mL for artesunate (AS). |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Artemisinin/Artesunate |
| Alternative Name | Artemisinin/Artesunate; Artemisinin (AM)/Artesunate (AS); AM/AS; Artemisinin; Artesunate; AM; AS |
