Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize APnEOs. The antibody showed a similar cross-reactivity with NPnEOs, nonylphenoxy carboxylates, and NPs, except for nonylphenoxyacetic acid and nonylphenol. It was established to quantitatively measure APnEOs in competitive enzyme-linked immunosorbent assay (ELISA). |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Non-species dependence |
Isotype | IgG1 kappa |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg |
Purification | Protein G affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | ELISA |
Application Notes | The reactivity of mabs and scFv antibodies was all examined in competitive ELISA and standard curves for NP10EO as a standard antigen. The IC50 values of mab MOF3-139 and the corresponding MOF3-139scFv were 27 ng/ml and 33 ng/ml, respectively. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | APnEOs |
Alternative Name | Alkylphenol polyethoxylate |