Description | This product is a monoclonal antibody derived from mouse by immunizing with an Nε-acetyllysine-containing simple peptide, GlyLys(Ac)-q-aminocaproic acid (Aca)-Cys, conjugated to KLH. The antibody was specific for Nε-acetyllysine, but not for Nα-acetyllysine and react with Nε-acetyllysine residues context independently to some extent. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Non-species dependence |
Immunogen | An Nε-acetyllysine-containing peptide, Gly-Lys(Ac)-ε-aminocaproic acid (Aca)-Cys, conjugated to KLH. |
Affinity | 10.7 nM for H4-5-GKG and 11.9 nM for p53-382-KKL |
Isotype | IgG1 kappa |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg |
Purification | Protein G affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | ELISA; WB |
Application Notes | The antibody is recommended for detection of Acetyllysine by ELISA, WB (1/1000-1/5000) assays. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | Acetyllysine |
Alternative Name | acetylated lysine |