| Description | This product is a monoclonal antibody which can be used to selectively bind to proteolytically active, high molecular weight ADAM10 protease. This antibody recognises a specific conformation of the ADAM10 substrate binding domain dependent on CxxC bonding. The mAb is capable of distinguishing tumour cells from normal or non-malignant cells. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Mouse |
| Epitope | The antibody binds to the CXXC motif of the active conformation of ADAM10. |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | IP; Inhib; WB; FuncS |
| Application Notes | Antibody clone 8C7 clearly inhibits signalling by Notch, and also Eph receptors, however the antibody does not interact directly with residues identified as contributing to substrate binding. The mAb recognises a specific conformation of the ADAM10 substrate binding domain dependent on CxxC bonding. 8C7 binding is blocked by mutation of the CxxC motif, and is altered by modulating the redox environment. mAb 8C7-immunoprecipitates of ADAM10 showed highly significant enrichment of protease activity. Also, oxidative conditions, known to enhance ADAM activity, favoured 8C7 binding. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ADAM10 |
| Alternative Name | RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717; disintegrin and metalloproteinase domain-containing protein 10; a disintegrin and metalloprotease domain 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease |
| Gene ID | 102 |
| UniProt | O14672 |
| Research Area | Cell Biology; Signal Pathway |
