| Description | This product is a human monoclonal antibody that specifically binds B7 family members PD-L1 protein. The antibody binds to the cytoplasmic domain of PD-L1. The cytoplasmic domain of PD-L1 is a 30-amino acid long chain that is unique to PD-L1, showing little homology to B7 family members. The antibody can be used for detecting surface expressed PD-L1. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | The antibody against the cytoplasmic domain of PD-L1 were generated. The amino acids sequence is: RLRKGRMMDVKKCGIQDTNSKKQSDTHLEET |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; IHC |
| Application Notes | The antibody is recommended for detection of CD274 by ELISA, IHC (1/20-1/200) assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
