Isotype controls are important negative controls and very important to reduce the overall false-positive results. Using an appropriate isotype control is critical for scientific research. It can help distinguish non-specific background stainings from specific antibody detection. Therefore, it can confirm staining results observed in immunoassays, such as flow cytometry (FACS), enzyme-linked immunosorbent assay (ELISA), western blot (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). Creative Biolabs provides a complete range of isotype controls covering common species, and with a variety of conjugates, such as Alexa Fluor® dyes, horseradish peroxidase (HRP), biotin, phycoerythrin (PE), fluorescein isothiocyanate (FITC), agarose, and other formats.
Isotype controls are antibodies that share similar properties with the primary antibodies but cannot achieve specific antibody-antigen binding. Used together with primary antibodies, negative controls help determine the existence of non-specific background staining. Generally, the isotype control should be negative when the background signal is unaffected by the Fc receptor binding, reactive epitopes, and endogenous enzymes.
Non-specific binding to primary antibodies is typically caused by: 1) Non-specific Fc receptor binding to cells. 2) Cell autofluorescence. 3) Non-specific antibody interactions with cellular proteins. Using the right isotype control can reduce background staining, allowing interpretation of the true signal. This allows a successful detection of target molecules. Including negative controls is, therefore, very important to help reduce the risk of false-positive results.
Creative Biolabs provides a comprehensive range of isotype controls formulated for both in vivo and in vitro applications. Advantages of our isotype controls including but not limited to:
For more detailed information, please feel free to contact us or send us an inquiry directly.