Secondary Antibodies

OVERVIEW

Together with primary antibodies, secondary antibodies are widely used as detection tools for target proteins in immunoassays. Creative Biolabs offers high-quality secondary antibodies that are generated in hosts such as chicken, donkey, goat, mouse, rabbit, rat, and sheep. Our secondary antibodies are validated and optimized in enzyme-linked immunosorbent assay (ELISA), western blot (WB), immunocytochemistry (ICC), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and more.

Secondary Antibodies

Tips for Choosing the Best Secondary Antibody

  • Host of the primary antibody
  • Secondary antibodies are produced against the host species of primary antibodies. So to determine the host species of the primary antibody is the first step. For instance, when using a primary antibody raised in a mouse, an anti-mouse secondary antibody should be brought from an alternative host such as sheep, rabbit, donkey, goat, or chicken.

  • Ig class and subclass of primary antibody
  • The desired secondary antibody must directly against the isotype of the primary antibody. For example, if the primary antibody is IgG, the secondary antibody should be anti-IgG that reacts directly to the heavy and light chain of the primary antibody. Secondary antibodies specific to IgG subclasses (such as anti-IgG1, anti-IgG2, anti-IgG2a, anti-IgG2b) are mainly used in double-labeling experiments, including ICC, IHC, and FC. If the primary antibody is subclass specific antibody, the secondary antibody must be subclass-specific. If the class/subclass of the primary antibody is unknown, we suggest using an anti-IgG Fab. Creative Biolabs offers a class of IgG antibodies, which are further classified into IgG1, IgG2a, IgG2b, and IgG3.

  • Conjugation
  • Secondary antibodies are normally conjugated for easy visualization. We offer a wide range of conjugates that cover the most wavelengths of fluorophore and enzymes. Conjugates for a specific secondary antibody may vary by the specific application. Common conjugates for secondary antibodies are horseradish peroxidase (HRP), biotin, alkaline phosphatase (AP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), Alexa Fluor series of dyes (Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 568, Alexa Fluor 594, and Alexa Fluor 647), DyLight series of dyes (DyLight 350, DyLight 488, DyLight 550, DyLight 650, and DyLight 800) and Cyanine Dyes (Cy2, Cy3, and Cy5) and rhodamine.

  • Desired purity of secondary antibody
  • Affinity-purified antibodies are isolated by separating monospecific antibodies by affinity chromatography. Advantages of using an affinity-purified antibody include increased specificity, lower background, greater sensitivity, and lot-to-lot consistency. A purified antibody will reduce variation which will yield more reproducible assays.

  • Final applications of secondary antibody
  • Secondary antibodies are widely used in research, clinical analysis, and disease diagnosis in all types of immunoassays, such as ELISA, WB, IHC and ICC, IP, and fluorescent in situ hybridization.

Advantages of Using Supreme Secondary Antibodies

  • Signal amplification
  • Increased accuracy, enhanced sensitivity, and improved results
  • No need to label primary antibodies
  • A primary antibody can be used with the same secondary antibody with different conjugations

With skilled scientists and years of experience in secondary antibody production, Creative Biolabs offers a wide range of secondary antibodies, best in the market but at a more competitive price. We also offer customized services to serve unique needs of our clients. For more detailed information, please feel free to contact us or send us an inquiry directly.

For research use only, not directly for clinical use.
TARGET SEARCH
A B C D F G H L M O P R S T
HOT TARGETS
• Alligator IgG (H+L) • Bat IgG (H+L) • Biotin • Bird IgG (H+L) • Bottlenose Dolphin IgG (H+L) • Bovine IgA • Bovine IgG (H+L) • Bovine IgG-Fab • Bovine IgG-heavy chain • Bovine IgG2 • Bovine IgM • Cat IgA • Cat IgG (H+L) • Cat IgG Fc • Cat IgG-Fab • Cat IgM • Cat Light Chain • Chicken IgA • Chicken IgM • Chicken IgY • Chicken IgY (H+L) • Dog IgA • Dog IgE • Dog IgG (H+L) • Dog IgG Fc • Dog IgG-Fab • Dog IgG1 • Dog IgM • Donkey IgG (H+L) • Ferret IgG (H+L) • Goat IgA • Goat IgG (H+L) • Goat IgG Fc • Goat IgG-Fab • Goat IgM • Guinea Pig IgG (H+L) • Hamster IgG (H+L) • Horse IgA • Horse IgG (H+L) • Horse IgG-Fab • Horse IgM • Human IgA • Human IgA1 • Human IgA2 • Human IgD • Human IgE • Human IgG • Human IgG + IgA + IgM • Human IgG Fc • Human IgG+IgM (H+L) • Human IgG+IgM+IgA (H+L) • Human IgG1 • Human IgG2 • Human IgG3 • Human IgG4-Fc • Human IgM • Human Kappa Light Chain • Human Lambda Light Chain • Llama IgG (H+L) • Monkey IgA • Mouse IgA • Mouse IgE • Mouse IgG (H+L) • Mouse IgG + IgM • Mouse IgG Fc • Mouse IgG+IgM+IgA (H+L) • Mouse IgG1 • Mouse IgG2a • Mouse IgG2a Kappa • Mouse IgG2c • Mouse IgG3 • Mouse IgM • Mouse IgM (H+L) • Mouse Kappa Light Chain • Mouse Lambda Light Chain • Opossum IgG (H+L) • Pig IgA • Pig IgG (H+L) • Pig IgG-Fab • Pig IgM • Rabbit IgA • Rabbit IgG • Rabbit IgG Fc • Rabbit IgM • Rat IgA • Rat IgG • Rat IgG (H+L) • Rat IgG+IgM+IgA (H+L) • Rat IgG1 • Rat IgG2a • Rat IgG2b • Rat IgG2c • Rat IgM • Sheep IgG (H+L) • Sheep IgG-Fab • Sheep IgG-heavy chain • Sheep IgM • Swine IgG (H+L) • Syrian Hamster IgG (H+L) • Turkey IgY (H+L)
VALIDATED PRODUCTS
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