Together with primary antibodies, secondary antibodies are widely used as detection tools for target proteins in immunoassays. Creative Biolabs offers high-quality secondary antibodies that are generated in hosts such as chicken, donkey, goat, mouse, rabbit, rat, and sheep. Our secondary antibodies are validated and optimized in enzyme-linked immunosorbent assay (ELISA), western blot (WB), immunocytochemistry (ICC), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and more.
Secondary antibodies are produced against the host species of primary antibodies. So to determine the host species of the primary antibody is the first step. For instance, when using a primary antibody raised in a mouse, an anti-mouse secondary antibody should be brought from an alternative host such as sheep, rabbit, donkey, goat, or chicken.
The desired secondary antibody must directly against the isotype of the primary antibody. For example, if the primary antibody is IgG, the secondary antibody should be anti-IgG that reacts directly to the heavy and light chain of the primary antibody. Secondary antibodies specific to IgG subclasses (such as anti-IgG1, anti-IgG2, anti-IgG2a, anti-IgG2b) are mainly used in double-labeling experiments, including ICC, IHC, and FC. If the primary antibody is subclass specific antibody, the secondary antibody must be subclass-specific. If the class/subclass of the primary antibody is unknown, we suggest using an anti-IgG Fab. Creative Biolabs offers a class of IgG antibodies, which are further classified into IgG1, IgG2a, IgG2b, and IgG3.
Secondary antibodies are normally conjugated for easy visualization. We offer a wide range of conjugates that cover the most wavelengths of fluorophore and enzymes. Conjugates for a specific secondary antibody may vary by the specific application. Common conjugates for secondary antibodies are horseradish peroxidase (HRP), biotin, alkaline phosphatase (AP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), Alexa Fluor series of dyes (Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 568, Alexa Fluor 594, and Alexa Fluor 647), DyLight series of dyes (DyLight 350, DyLight 488, DyLight 550, DyLight 650, and DyLight 800) and Cyanine Dyes (Cy2, Cy3, and Cy5) and rhodamine.
Affinity-purified antibodies are isolated by separating monospecific antibodies by affinity chromatography. Advantages of using an affinity-purified antibody include increased specificity, lower background, greater sensitivity, and lot-to-lot consistency. A purified antibody will reduce variation which will yield more reproducible assays.
Secondary antibodies are widely used in research, clinical analysis, and disease diagnosis in all types of immunoassays, such as ELISA, WB, IHC and ICC, IP, and fluorescent in situ hybridization.
With skilled scientists and years of experience in secondary antibody production, Creative Biolabs offers a wide range of secondary antibodies, best in the market but at a more competitive price. We also offer customized services to serve unique needs of our clients. For more detailed information, please feel free to contact us or send us an inquiry directly.