This product is a human monoclonal antibody that reacts with CD4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
P815 mastocytoma ce11stransfected with human CD4 cDNA
HEK293F or CHO
>95%, determined by SDS-PAGE and/or SEC-HPLC
<1 EU/mg, determined by LAL method
Protein A affinity purified
0.2 μM filtered
PBS, pH 7.4
Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.
WB; FuncS; IF; Neut; ELISA; FC; IP
The antibody is recommended for detection of CD4 by WB, FuncS, IF, Neut, ELISA, FC, IP assays.