| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for CD47 Molecule. It can be used for CD47 Molecule detection in Enzyme-Linked Immunosorbent Assay and Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Affinity | The affinity is in picomolar range, about 40 pM, determined by surface plasmon resonance. |
| Isotype | IgG2 kappa |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD47 |
| Alternative Name | CD47 Molecule; Antigenic Surface Determinant Protein OA3; CD47 Antigen (Rh-Related Antigen, Integrin-Associated Signal Transducer); Antigen Identified By Monoclonal Antibody 1D8; Integrin Associated Protein; Integrin-Associated Protein; Rh-Related Antigen; CD47 Glycoprotein; MER6; IAP; Integrin-Associated Signal Transducer; Leukocyte Surface Antigen CD47; CD47 Antigen; Protein MER6; OA3 |
| Gene ID | 961 |
| UniProt | Q08722 |
| Research Area | Immunology; Cancer Research |
| Related Disease | Cancer and other neoplastic disorder |
