Recombinant Anti-CD80 Antibody (V3S-1022-YC2322) (CAT#: V3S-1022-YC2322)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant Monkey antibody provided by CreativeBiolabs. The antibody is specific for CD80 Molecule. It can be used for CD80 Molecule detection in Flow Cytometry and Enzyme-Linked Immunosorbent Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Monkey
Target Species Human
Affinity The affinity is in nanomolar range, about 11 nM, determined by surface plasmon resonance.
Isotype IgG

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application FC; ELISA

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CD80
Alternative Name CD80 Molecule; CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen); B-Lymphocyte Activation Antigen B7; CTLA-4 Counter-Receptor B7.1; Activation B7-1 Antigen; CD28LG1; CD28LG; LAB7; BB1; B7; Costimulatory Molecule Variant IgV-CD80; Costimulatory Factor CD80; CD80 Antigen; B7-1; B7.1
Gene ID 941
UniProt P33681
Research Area Immunology; Stem Cell
Related Disease Crohn's disease
For research use only, not directly for clinical use.
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