| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Cathepsin D. It can be used for Cathepsin D detection in Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunoprecipitation and Neutralization Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Epitope | The epitope is in the 128AAKFDG134, 172DPDAQPGG179 located on the 34 kDa Cath-D. |
| Affinity | The affinity is in nanomolar range, about 100 ± 30 nM, determined by surface plasmon resonance. |
| Isotype | IgG1 lambda |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; IF; IP; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CTSD |
| Alternative Name | Cathepsin D; EC 3.4.23.5; CPSD; Epididymis Secretory Sperm Binding Protein Li 130P; Cathepsin D (Lysosomal Aspartyl Protease); Ceroid-Lipofuscinosis, Neuronal 10; Lysosomal Aspartyl Peptidase; Lysosomal Aspartyl Protease; Ceroid-Lipofuscinosis; Neuronal 10; HEL-S-130P; EC 3.4.23; CLN10 |
| Gene ID | 1509 |
| UniProt | P07339 |
| Research Area | Neuroscience; Cancer Research |
| Related Disease | Cancer |
