| Description | This product is a human nuetralizing antibody clone CAP257-RH1, which binding Human immunodeficiencyvirus-1 glycoprotein 120 (HIV-1 gp120). It is an N276 glycan-dependent CD4 binding site antibody. It could potently neutralize HIV-1 virus strain. This product has been detected using Enzyme-linked immunosorbent assay and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | HIV1 |
| Epitope | N276 glycan-dependent epitope |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| Application Notes | ELISA: Serial dilutions of antibody in 5% skim milk powder in PBS were added for 1 h at 37°C. Neutralization assay: CAP257-RH1 potently neutralized heterologous tier 2 clade B viral strain RHPA with an IC50 of 0.11 μg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV1 gp120 |
| Alternative Name | Human immunodeficiencyvirus-1 glycoprotein 120 |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
