| Description | This antibody is a mouse neutralizing antibody clone M4H2K1 binding with HIV-1 gp120 envelope protein. It recognized the C3/V4 region of the native-like BG505 Env. It appeared to be one of the most potent neutralizing antibodies that interacts with HIV-1 gp120. This product has been tested using Enzyme-linked immunosorbent assay and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | HIV1 |
| Immunogen | Protein NP |
| Epitope | C3/V4 region on HIV1 BG505 strain Env |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| Application Notes | ELISA: The antibody started at 10 μg/mL with a serial 1:10 dilution. Neutralization assay: The IC50 of M4H2K1 to HIV-1 is 0.067 μg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV1 gp120 |
| Alternative Name | Human immunodeficiencyvirus-1 BG505 Envelope |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
