| Description | The antibody 01.4B is a human IgG specific for Respiratory syncytial virus. It interacts with β3-β4 and the base of the α4 helix in site V as well as residues 262-265 associated with antigenic site II. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Respiratory syncytial virus |
| Affinity | 01.4B displayed high affinity (10.5 nM) for DS-Cav1. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | mAbs were biotinylated using an EZ-Link sulfo-NHS-biotinylation kit and titrated on DS-Cav1-coated plates. Avidin D-HRP conjugate and TMB were used for color development. The optical density at 450 nm was determined with SpectraMax Plus. The concentration of biotinylated mAb in the linear range of the titration curve was chosen for competition ELISA. Unlabeled competitor mAbs were serially diluted and added to the DS-Cav1-coated plate. Following incubation for 30 min at room temperature, biotinylated mAbs were added, and OD readings were recorded using biotinylated mAb alone as a binding control. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | Respiratory syncytial virus |
| Alternative Name | Respiratory syncytial virus |
| Research Area | Infectious Disease |
| Related Disease | Pneumonia; Middle ear infection; Asthma |
