Anti-Respiratory syncytial virus pre-F Neutralizing Antibody (V3S-0623-XY3) (CAT#: V3S-0623-XY3)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description The anti-Respiratory syncytial virus antibody 32.4K is a human IgG. It mainly uses its CDR H3 to bind to respiratory syncytial virus pre-F.
Clonality Monoclonal
Host Species Human
Target Species Respiratory syncytial virus
Epitope Antibody 32.4K bound to antigenic site Ø of pre-F.
Affinity The binding affinity of 32.4K to DS-Cav1 was 1.1 nM.
Isotype IgG1

Property

Expression Species HEK293F or CHO cell line
Conjugation Unconjugated
Purity >95%
Endotoxin <1 EU/mg
Form Liquid
Purification Protein A purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA, Neut
Application Notes mAbs were biotinylated using an EZ-Link sulfo-NHS-biotinylation kit and titrated on DS-Cav1-coated plates. Avidin D-HRP conjugate and TMB were used for color development. The optical density at 450 nm was determined with SpectraMax Plus. The concentration of biotinylated mAb in the linear range of the titration curve was chosen for competition ELISA. Unlabeled competitor mAbs were serially diluted and added to the DS-Cav1-coated plate. Following incubation for 30 min at room temperature, biotinylated mAbs were added, and OD readings were recorded using biotinylated mAb alone as a binding control.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Western Blot Protocol

Target

Target Respiratory syncytial virus
Alternative Name Respiratory syncytial virus
Research Area Infectious Disease
Related Disease Pneumonia; Middle ear infection; Asthma
For research use only, not directly for clinical use.
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