| Description | The antibody CV30 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It binds almost exclusively to the concave ACE2-binding epitope of the RBD using all six complementary determining region (CDR) loops. It neutralizes by blocking ACE2 binding and inducing S1 shedding. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Receptor-binding domain (RBD) |
| Function | Blocking ACE2 binding and inducing S1 shedding. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | Immulon 2HB microtiter plates were coated with 50 ng/well of SARS-CoV-1 or SARS-CoV-2 RBD at 4°C overnight. Plates were washed 4X with PBS with 0.02% Tween-20. Plates were blocked with 250 μL of 10% non-fat milk and 0.02% Tween-20 in PBS (blocking buffer) for 1 h at 37°C. After washing 4× with wash buffer, purified IgG was added at an initial concentration of 20 μg/mL and diluted in tenfold serial dilutions in blocking buffer and incubated for 1 h at 37°C. Plates were washed 4× in wash buffer and the secondary antibody Goat anti-Human Ig-HRP was added at a 1 : 3000 dilution and incubated at 37°C for 1 h. After a final 4× wash, 50 μL of SureBlue Reserve TMB Peroxidase Substrate was added and incubated for 4 min followed by addition of 100 μL of 1 N H2SO4 to stop the reaction. The optical density at 450 nm was measured using a SpectraMax M2 plate reader. All wash steps were performed using a BioTek 405 Select Microplate Washer. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
