| Description | The antibody FD-11A is a human mAb specific for SARS-CoV-2 Spike S1 protein. It targets the right hip of the RBD (right shoulder in the torso analogy). It partially inhibited the interaction of RBD with ACE2 through steric hindrance. It interferes with the RBD-ACE2 interaction and prevent the spike protein-mediated cell-cell fusion. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Receptor-binding domain (RBD) |
| Function | Interferes with the RBD-ACE2 interaction and prevent the spike protein-mediated cell-cell fusion. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | The NUNC plate was coated with recombinant RBD proteins in carbonate buffer and incubated at 4°C for 16 h. Nonspecific binding was blocked with the solution of 3% bovine serum albumin for 1 h at room temperature on a shaker. After removing the blocking buffer, the plate was incubated with serial dilutions of the antibody for 60 m at 37°C. After washing, the plate was incubated with horseradish peroxidase-conjugated anti-human or anti-mouse IgG for 60 min at room temperature. After washing, the plate was developed with TMB substrate reagent. The reaction was stopped by 0.5 M hydrochloric acid and the optical density was measured at OD450 on a microplate reader. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
