| Description | The antibody FI-3A is a human mAb specific for SARS-CoV-2 Spike S1 protein. It targets the head and neck (left shoulder of the torso) of the RBD and are typical neutralizing antibodies that block the binding of SARS-CoV-2 RBD to ACE2 protein. It interferes with the RBD-ACE2 interaction and prevent the spike protein-mediated cell-cell fusion. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Receptor-binding domain (RBD) |
| Function | Interferes with the RBD-ACE2 interaction and prevent the spike protein-mediated cell-cell fusion. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | The NUNC plate was coated with recombinant RBD proteins in carbonate buffer and incubated at 4°C for 16 h. Nonspecific binding was blocked with the solution of 3% bovine serum albumin for 1 h at room temperature on a shaker. After removing the blocking buffer, the plate was incubated with serial dilutions of the antibody for 60 m at 37°C. After washing, the plate was incubated with horseradish peroxidase-conjugated anti-human or anti-mouse IgG for 60 min at room temperature. After washing, the plate was developed with TMB substrate reagent. The reaction was stopped by 0.5 M hydrochloric acid and the optical density was measured at OD450 on a microplate reader. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
