| Description | The mAb CV3-25 is a monoclonal antibody against Spike S2 protein of SARS-CoV-2. It neutralizes SARS-CoV-2 variants and a SARS-like bat coronavirus. It binds to a linear epitope on the SARS-CoV-2 stem helix. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | SARS-CoV-2 variants, SARS-like bat coronavirus |
| Epitope | A linear epitope on the SARS-CoV-2 stem helix. |
| Function | Neutralizes SARS-CoV-2 variants and a SARS-like bat coronavirus |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | MaxiSorp microtiter plates were coated with 300 ng/well of streptavidin overnight at room temperature. Plates were washed 4X with PBS with 0.02% Tween-20, then incubated with 60 μL/well of 3% BSA and 0.02% Tween-20 in PBS for 1 hr at 37°C. After washing 4X with wash buffer, 380 ng/well of biotinylated peptides diluted in blocking buffer were incubated for 1 hr at 37°C. Plates were washed 4X in wash buffer and then mAbs were serially diluted in blocking buffer, added to the plate and incubated for 1 hr at 37°C. Plates were washed 4X in wash buffer and the secondary antibody Goat anti-Human Ig-HRP, was added and incubated at 37°C for 1 hr. Plates were washed 4X wash buffer, and then 30 μL/well of SureBlue Reserve TMB Per_x0002_oxidase Substrate was added and incubated for 3 min followed by addition of 30 μL of 1 N H2SO4 to stop the reaction. The optical density at 450 nm was measured using a SpectraMax i3x plate reader. All wash steps were performed using a BioTek 405/TSMicroplate Washer. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
