Description | This product is a human monoclonal antibody that reacts with SLC40A1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human |
Immunogen | 293T cells transiently expressing ferroportin or with membrane preparations from 293E6 cells expressing ferroportin |
Isotype | IgG |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Protein A affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
Application | ELISA; WB |
Application Notes | The antibody is recommended for detection of SLC40A1 by ELISA, WB assays. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | SLC40A1 |
Alternative Name | SLC40A1; solute carrier family 40 (iron-regulated transporter), member 1; FPN1; HFE4; MTP1; IREG1; MST079; MSTP079; SLC11A3; solute carrier family 40 member 1; iron regulated gene 1; putative ferroportin 1 variant IIIA; putative ferroportin 1 variant IIIB; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3 |
Gene ID | 30061 |
UniProt | Q9NP59 |
Research Area | Signal Pathway |