| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Sortilin 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Epitope | The epitope is in the peptides correspond to amino acid residues 237-247 and 314-338 and has the amino acid sequences of: NGLWVSKNFGG and FASVMADKDTTRRIHVSTDQGDTWS. Further, the cross-linked residues within the peptides correspond to residues S242 and K243 within amino acid residues 237-247 and residues S316 and R325 within amino acid residues 314-338. |
| Affinity | KD = 0.23 nM, determined by surface plasmon resonance. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; Block |
| Application Notes | The antibody is recommended for detection of SORT1 by ELISA, FC, Block assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
