| Description | The 5F10 clone is a human recombinant antibody that specifically targets Human CD274. This 5F10 monoclonal antibody has the ability to inhibit the interaction between PD-1 and PDL-1. In terms of affinity, the mouse antibody 5F10 demonstrates 0.029 nM in ELISA, 0.838 nM in competitive ELISA, and 1.48 nM in cell affinity for detection. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1, kappa |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC |
| Application Notes | The mouse antibody 5F10 demonstrates 0.029 nM in ELISA, 0.838 nM in competitive ELISA, and 1.48 nM in cell affinity for detection. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
