| Description | This recombinant anti-DOA9 antibody (clone bDOA9rb8) specifically recognizes Bradyrhizobium DOA9 O-antigen. In both free-living cells (pure culture) and bacteroids inside plant nodules, DOA9 shows no cross-reactivity with other related bradyrhizobial strains, i.e., USDA110, SUTN9-2, and SUTN1-12, or Bacillus sp. |
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Target Species | Bradyrhizobium sp. DOA9 |
| Immunogen | DOA9 cell antigen |
| Epitope | Bradyrhizobium sp. O-antigen |
| Isotype | Rabbit IgG1, λ |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; IF |
| Application Notes | The binding property of scFv bDOA9rb8 was demonstrated by ELISA. The results of ELISA and Immunofluorescence assay showed that this antibody had no cross-reactivity with other related bradyrhizobial strains, i.e., USDA110, SUTN9-2, and SUTN1-12, or Bacillus sp.(only ELISA). Purity of the scFv was analyzed by Western Blot. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | DOA9 O-antigen |
| Alternative Name | O-antigen; O antigen |
| Research Area | Microbiology |
