Recombinant Anti-SARS-CoV-2 Neutralizing Antibody (V3S-0523-XY85) (CAT#: V3S-0523-XY85)

Send Inquiry
  • fig1

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a neutralizing antibody that binds to Severe Acute Respiratory Syndrome Coronavirus 2. DH1041 is capable of specifically binding with SARS-CoV-2 and shows a good capacity to neutralize the SARS-CoV-2.
Clonality Monoclonal
Host Species Human
Target Species SARS-CoV-2
Epitope RBD receptor binding domain
Isotype IgG1

Property

Expression Species HEK293F or CHO cell line
Conjugation Unconjugated
Purity >95%
Endotoxin <1 EU/mg
Form Liquid
Purification Protein A purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA
Application Notes For binding ELISA, 384-well ELISA plates were coated with 2 μg/mL of antigens in 0.1 M sodium bicarbonate overnight at 4_x005f_x0004_°C. Plates were washed with PBS + 0.05% Tween 20 and blocked with blocked with assay diluent at room temperature for 1 h. Purified mAb samples in 3-fold serial dilutions in assay diluent starting at 100 mg/mL, or un-diluted transfection supernatant were added and incubated for 1 hour, followed by washing with PBS-0.1% Tween 20. HRP-conjugated goat anti-human IgG secondary Ab was diluted to 1:10,000 and incubated at room temperature for 1 h. These plates were washed four times and developed with tetramethylbenzidine substrate. The reaction was stopped with 1 M HCl, and optical density at 450 nm was determined.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target SARS-CoV-2
Alternative Name Severe acute respiratory syndrome coronavirus 2
Research Area Coronavirus Disease 2019
Related Disease Coronavirus Disease 2019
For research use only, not directly for clinical use.
banner banner
© 2024 Creative Biolabs. All Rights Reserved.
antibody
Online Inquiry