| Description | This product is a neutralizing antibody that binds to Severe Acute Respiratory Syndrome Coronavirus 2. P2B-2F6 is capable of specifically binding with SARS-CoV-2 and shows a good capacity to neutralize the SARS-CoV-2. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | RBD receptor binding domain |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA |
| Application Notes | Nunc MaxiSorp plates were coated with 100 μL of purified trimeric FL-spike ectodomain (0.01 mg/ml) diluted in 50 mM Na-carbonate (pH 9.5). Plates were incubated overnight at 4°C and then washed three times with PBST. Plates were blocked for 1 hour at room temperature with 2% BSA in PBST and then washed three times with PBST. Serum was diluted in 2% BSA in PBST, and 100 μL was added to each well. After 1 hour of incubation at room temperature, plates were washed three times with PBST and 200 μL of 1:5000 peroxidase-conjugated anti-mouse IgG was added. Plates were incubated for 30 min at room temperature and washed three times with PBST. Last, 70 μL of TMB was added and incubated for 20 min at room temperature before quenching with 70 μL of 2 M H2SO4. Abs450 was measured using a Biotek Synergy H1 plate reader. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
