Description | This is a mouse antibody that is specific to human two-chain hepatocyte growth factor (tcHGF). tcHGF can activate the MET receptor and single-chain HGF (scHGF) is inactive. The monoclonal antibody exclusively recognizes tcHGF, while it recognizes human HGF but not mouse HGF. The anti-human HGF monoclonal antibody is used in IHC applications with a dilution of 1/500. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Human |
Isotype | Mouse IgG |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg |
Purification | Protein G purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | ELISA; IHC; WB |
Application Notes | IHC: 1/500 WB: 1/1000 |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | TcHGF |
Alternative Name | Two-chain HGF; active tcHGF; SF; HGFB; HPTA; F-TCF; DFNB39; hepatocyte growth factor; fibroblast-derived tumor cytotoxic factor; hepatocyte growth factor (hepapoietin A; scatter factor); hepatopoietin-A; lung fibroblast-derived mitogen |
Gene ID | 3082 |
UniProt | P14210 |
Research Area | Signal Pathway |