Anti-APP Neutralizing Antibody (V3S-0622-YC4117) (CAT#: V3S-0622-YC4117)

Figure 1 Anti-APP Monoclonal Antibody (V3S-0622-YC4117) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC4117 in non-reduced (lane 1, 2.5 μg) and reduced (lane 2, 2.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Anti-APP Monoclonal Antibody (V3S-0622-YC4117) in SEC-HPLC

The purity of V3S-0622-YC4117 was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Anti-APP Monoclonal Antibody (V3S-0622-YC4117) in WB

Western blot analysis was performed by loading 1 µg (lane 1, Non-Reduced) and 1 µg (lane 2, Reduced) recombinant human Beta-amyloid 42 with His & GST Tag (10703-H20E2) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human Abeta Antibody (V3S-0622-YC4117) at a concentration of 1 μg/mL overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.

Figure 4 Anti-APP Monoclonal Antibody (V3S-0622-YC4117) in ELISA

ELISA analysis of V3S-0622-YC4117 was performed by coating with recombinant human Beta-amyloid 42 with His & GST Tag (10703-H20E2) (1 μg/mL). Then blocked with BSA and incubated with Anti-Human APP Antibody (V3S-0622-YC4117) at a starting concentration of 2.5 ng/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.