| Description | This product is a human monoclonal antibody that reacts with ASGR1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Mouse |
| Immunogen | Mouse ASGPR |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; FuncS |
| Application Notes | The antibody is recommended for detection of ASGR1 by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ASGR1 |
| Alternative Name | ASGR1; asialoglycoprotein receptor 1; HL-1; ASGPR; ASGPR1; CLEC4H |
| Research Area | Cell Biology |
Figure 1 Anti-Mouse ASGR Monoclonal Antibody (V3S-0622-YC967) scFv Fragment with C-term Cysteine in WB
Western blot analysis of V3S-0622-YC967 scFv fragment was performed by loading Recombinant mouse ASGR1 (His Tag).
V3S-0622-YC967 incubation concentration: 4 ng/μL.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
Figure 1 Anti-Mouse ASGR Monoclonal Antibody (V3S-0622-YC967) scFv Fragment with C-term Cysteine in WB
