| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Bacillus anthracis lethal factor (LF). It can be used for B. anthracis LF detection in Enzyme-linked Immunosorbent Assay (ELISA), Neutralization (Neut). It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Bacillus anthracis |
| Immunogen | Bacillus anthracis Lethal Factor (LF) |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | B. anthracis LF |
| Alternative Name | Anthrax lethal toxin endopeptidase component; Anthrax LF; Lef; Lethal factor; LF |
| Research Area | Microbiology |
| Related Disease | Anthrax (Bacillus anthracis) infection |
