| Description | This product is a human monoclonal antibody that reacts with B5 Env.The mAb displayed high binding affinities to B5 (Kd of 0.7 nM). The monoclonal anti-B5 clone reduced the formation of cometlike plaques of VACV EV. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Vaccinia Virus (VACV) |
| Affinity | 0.7 nM |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; Neut; FuncS |
| Application Notes | In vitro neutralizing activity BS-C-1 cells were infected with about 50 pfu of VACV strain IHD-J. After 2 hours at 37°C, the monolayer was washed, and fresh medium containing indicated amounts of chimpanzee anti-B5 8AH8AL was added. After 48 hours, the monolayers were stained with crystal violet. For the smallpox assay (b), monolayers of BS-C-40 cells in six-well cell culture plates were infected with the Solaimen strain of variola virus at 50 pfu per well in RPMI medium containing 2% FBS. After 1 hour, the medium was aspirated; cells were washed twice and overlaid with RPMI medium containing 25, 2.5, or 0 ug of anti-B5 IgG. The plates were then incubated in a CO2 incubator for 4 days at 35.5°C. Cells were fixed and reacted with polyclonal rabbit anti-variola virus antibody. After incubation with goat anti-rabbit-HRP conjugate, comets were visualized by addition of TrueBlue peroxidase substrate. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | B5 Env |
| Alternative Name | B5 envelope glycoprotein; B5; VACV; Vaccinia virus |
| Research Area | Infectious Disease |
Figure 1 Recombinant Anti-VACV B5 GP Monoclonal Antibody in WB.
Western blot analysis of V3S-0622-YC39 was performed by loading VACV B5 Protein.
Figure 1 Recombinant Anti-VACV B5 GP Monoclonal Antibody in WB.
Figure 2 Recombinant Anti-VACV B5 GP Monoclonal Antibody in ELISA.
ELISA analysis of V3S-0622-YC39 was performed by coating with VACV B5 Protein.
Figure 2 Recombinant Anti-VACV B5 GP Monoclonal Antibody in ELISA.
