| Description | This product is a monoclonal antibody derived from rat, which can specifically recognize BAK1. The antibody can change the conformation of Bak from a non-activated to an activated conformation. It is proved that Incubating the anti-Bak antibody with mitochondria promoted full activation of Bak and resulted in cytochrome c release. The antibody was as effective as the potent natural activator tBid in eliciting Bak conformation change and oligomerisation. |
| Clonality | Monoclonal |
| Host Species | Rat |
| Target Species | Human |
| Epitope | The antibody bound peptides in the a 1 -2 loop. |
| Affinity | 4.3 nM |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Activ |
| Application Notes | The antibody is recommended for detection of BAK1 by ELISA, Activ assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
