| Description | This is a human IgG1, λ monoclonal antibody designed to target BoNT/A. It is suitable for use in ELISA, Neut, WB, and Inhib assay. The antibody exhibited a 92% inhibition of the cleavage induced by BoNT/A. Through ELISA, it was observed that the antibody specifically binds to the BoNT/A LC, and not to the BoNT/A heavy chain. Furthermore, when administered alone, the antibody demonstrated potent in vivo neutralization, and in collaboration with an antibody specific for the HC. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | C. botulinum |
| Neutralization Potency | An equimolar mix of 6A and 4LCA antibodies neutralized BoNT/A with a potency of 10,000 LD50/mg antibody. |
| Affinity | KD = 31 pM |
| Isotype | Human IgG1, lambda |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Neut; WB; Inhib |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | BONT-A |
| Alternative Name | Botulinum Toxin Type A; BONT-A |
| Research Area | Microbiology |
