| Description | This product is a human monoclonal antibody against the C-antigen that is found specifically on neoplastic cells. The clone specifically recognizes cancer cells from a wide variety of cancers cell lines (e.g. breast adenocarcinoma, malignant melanoma, neuroblastoma, glioblastoma, colon adenocarcinoma, small cell lung carcinoma, lung adenocarcinoma and prostate adenocarcinoma) but does not recognize normal, non-cancerous cells. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgM kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | FC; ELISA; IP; IHC |
| Application Notes | Cell-fixed ELISA Growing tumor cells were collected by centrifugation, washed with PBS, and 50 μl of cell suspension containing 5,000-10,000 cells placed in each well of 96-well ELISA plates. After allowing the cells to attach to the plates, the culture supernatants were removed and the plates were blocked with PBS-BSA. The cells were then incubated with different concentrations (1-20 μg/mL) of either H11 or control human myeloma IgM for 2 hrs. The plates were washed and incubated with biotin-conjugated goat anti-human IgM. Then the plate were incubated with streptavidin-conjugated alkaline phosphatase and p-nitrophenyl phosphate substrate and read at 405 nm in an ELISA plate reader. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | C-antigen |
| Alternative Name | C-Antigen; Neoplastic Cells; H11; MAb H11; NBGM1/H11 |
