| Description | This product is a human monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing c-Met. It can be used for c-Met detection in Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry (FC), Neutralization Assay (Neut), Functional Assay (FuncS). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Monkey |
| Epitope | The antibody appears to bind a conformational epitope comprising four regions of the c-Met Sema domain, located in the α-chain of the human c-Met extracellular domain (amino acid residues 25-307). |
| Isotype | IgG4 kappa |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A or G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; Neut; FuncS |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | c-Met |
| Alternative Name | Tyrosine-protein kinase Met; hepatocyte growth factor receptor (HGFR) |
| Research Area | Cell Biology |
| Related Disease | Cancer |
