| Description | This product is a humanized monoclonal antibody that binds to free human C5a with high affinity and, with a much lower affinity, to uncleaved human C5. The antibody is cross-reactive with C5a from non-human primate species including cynomolgus (33 nM) and mouse (0.4 nM). |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Cynomolgus, Mouse |
| Immunogen | Human desarginated C5a |
| Affinity | BNJ383 bound to human C5a with a KD less than 100 picomolar. |
| Isotype | IgG2 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib; FuncS |
| Application Notes | The humanized antibody was subjected to BIAcore analysis to quantify their respective affinities for human C5a. Briefly, the mAb was screened with 3-4 concentrations of human C5a (antigen) using a capture technique. The antibody was captured by an Anti-Fc (human) directly immobilized on a CM5 sensor chip with various concentrations in the range from 0.6 nM to 5.9 nM of human C5a passed over the sensor chip surface. To evaluate the efficacy of an anti-C5a antibody to inhibit hC5a-induced neutropenia in mice, different dosages (24 mg/kg, 12 mg/kg, 6 mg/kg, and 3 mg/kg) of BNJ383 were administered to Balb/c mice 24 hours prior to hC5a injection. Administration of the anti-C5a antibody 24 hours ahead of hC5a allowed for the pharmacodynamics properties of the antibody to be studied during the β-phase of antibody elimination from the mice. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | C5a |
| Alternative Name | C5; Complement C5 alpha chain; C5D; C5a; C5b; ECLZB; CPAMD4; complement C5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C5a anaphylatoxin; anaphylatoxin C5a analog; complement component 5; prepro-C5 |
| Gene ID | 727 |
| UniProt | P01031 |
| Research Area | Immunology |
