| Description | A fully human monoclonal antibody that specifically binds to the chemokine regulated upon activation, normal T-cell expressed, and secreted (RANTES, CCL5). 1E4 inhibits the activity of both recombinant and native huRANTES in a dose-dependent manner. 1E4 was able to inhibit calcium flux in cells that express either hCCR1, hCCR3 or hCCR5. 1E4 only binds to humans and cynomolgus RANTES and not with RANTES from other species nor with any of the other human chemokines. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Cynomolgus |
| Epitope | Residues A16, R17 and P18 are critical for the antibody binding. |
| IC50 | 1.25 nM |
| Affinity | KD = 0.45 nM (Human), KD = 2.24 nM (Cynomolgus monkey). |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib |
| Application Notes | ELISA: 1 μg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CCL5 |
| Alternative Name | C-C Motif Chemokine Ligand 5; SIS-Delta; Regulated Upon Activation, Normally T-Expressed, And Presumably Secreted; Small Inducible Cytokine Subfamily A (Cys-Cys), Member 5; Eosinophil Chemotactic Cytokine; Chemokine (C-C Motif) Ligand 5; T-Cell Specific Protein P288; Beta-Chemokine RANTES; D17S136E; TCP228; SCYA5 |
| Gene ID | 6352 |
| UniProt | P13501 |
| Related pathway | PI3K/AKT, NF-κB, ERK/MEK and HIF signaling pathways |
| Research Area | Autophagy; Signal Pathway |
| Related Disease | Autoimmune disease or inflammatory disorder |
