| Description | This product is a humanized anti-CCR5 antibody which minimized the potential for immunogenicity. ST6/34 is shown to efficiently prevented cell surface expression of CCR5 expressed intracellularly with an endoplasmic reticulum retention signal. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Purified CCR5-N-GST protein |
| Epitope | The epitope is located in the amino acids region 14-19 (YYTSEP). |
| Affinity | 8.5 nM |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Block |
| Application Notes | Surface Plasmon Resonance Surface plasmon resonance of rabbit Fab ST6 and humanized Fab ST6/34 to CCR5-N-MBP antigen was performed on a Biacore instrument. Binding of Fab to immobilized CCR5-N-MBP was studied by injection of Fab at five different concentrations ranging from 50 to 150 nM. Activation, deactivation, regeneration of the CM5 sensor chip and evaluation of obtained data were done. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CCR5 |
| Alternative Name | CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5; C-C chemokine receptor type 5; C-C motif chemokine receptor 5 A159A; HIV-1 fusion coreceptor; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemokine recptor CCR5 Delta32; chemr13 |
| Gene ID | 1234 |
| UniProt | P51681 |
| Research Area | Neuroscience; Immunology |
