| Description | This product is a blocking anti-CD22 monoclonal antibody specifically binding to the first two Ig-like domains of native human CD22 (hCD22). The antibody HB22-23 completely blocked (80-100%) the binding of Daudi, Raji and Jurkat cells to CD22 transfected COS cells. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | A mouse pre-B cell line, 300.19, stably transfected with a full-length CD22 cDNA. |
| Epitope | The epitope is located in the first two Ig-like domains. |
| Isotype | IgG2a kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | Block |
| Application Notes | The antibody is recommended for detection of CD22 by Block assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD22 |
| Alternative Name | CD22 Molecule; CD22 Antigen; Sialic Acid-Binding Ig-Like Lectin 2; B-Lymphocyte Cell Adhesion Molecule; T-Cell Surface Antigen Leu-14; SIGLEC-2; HB-11349 |
| Gene ID | 933 |
| UniProt | P20273 |
| Related pathway | Hematopoietic Stem Cell Differentiation Pathways; Lineage-specific Markers; Innate Immune System |
| Research Area | Immunology; Cancer Research |
