Anti-CD274 Neutralizing Antibody (V3S-0522-YC1445) (CAT#: V3S-0522-YC1445)

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Datasheet

MSDS

COA

Sub Cat Target Species Isotype Application Affinity  
V3S-1124-YC637 Human IgG1 kappa ELISA; IHC; FC; IP; IF; Block Inquiry
V3S-0522-YC3114 Human, Mouse IgG ELISA; FC; Block 0.162 nM Inquiry
V3S-0522-YC3115 Human, Mouse IgG1 ELISA; FC; Block 0.963 nM Inquiry
V3S-0522-YC3127 Human IgG1 ELISA; FC; Block; FuncS 80.8 pM Inquiry
V3S-0522-YC3133 Human IgG ELISA; FC; Block 6.355 nM Inquiry
V3S-0522-YC3134 Human IgG ELISA; FC; Block 43.17 nM Inquiry
V3S-0522-YC3135 Human IgG ELISA; FC; Block 65.43 nM Inquiry
V3S-0522-YC3139 Human, Cynomolgus IgG FC; Block 0.847 nM Inquiry
V3S-0522-YC3140 Human, Cynomolgus, Mouse IgG kappa FC; Block 1 pM Inquiry
V3S-0522-YC8182 Human IgG Neut; FuncS Inquiry
V3S-0622-YC1249 Human IgG FuncS Inquiry
V3S-0822-YC688 Human, Mouse IgG1 kappa Neut Inquiry
V3S-0822-YC689 Human IgG1 kappa ELISA; Neut Inquiry
V3S-0522-YC3110 Human IgG ELISA; FC; Block Inquiry
V3S-0522-YC3119 Human IgG1 Block; FuncS Inquiry
V3S-0522-YC3125 Human IgG ELISA; FC; Inhib Inquiry
V3S-0822-YC2771 Human IgG2b kappa Block; FuncS; IHC-Fr; FC Inquiry
V3S-0822-YC687 Human, Rat, Chimpanzee IgG2b kappa IHC-P; FC; Neut; ICC Inquiry
V3S-0822-YC690 Human IgG1 kappa FC; FuncS; Neut; IHC; IF Inquiry
V3S-1022-YC395 Human IgG ELISA; Block 2.33 nM Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Programmed cell death 1 ligand 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgG1, κ

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; IHC; FC; IP; IF; Block
Application Notes The antibody is recommended for detection of CD274 by ELISA, IHC, FC, IP, IF, Block assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CD274
Alternative Name Programmed cell death 1 ligand 1; B7H1; B7-H1; PDL1; PD-L1; PDCD1L1; B7 homolog 1; B7 homologue 1
Gene ID 29126
UniProt Q9NZQ7
Research Area Immunology
Related Disease Cancers, non-small cell lung (NSCLC), Solid tumors, bladder, urothelial
For research use only, not directly for clinical use.
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