Recombinant Anti-CD276 (ECD domain) Antibody (V3S-1022-YC2513) (CAT#: V3S-1022-YC2513)

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Description This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for CD276 Molecule. It can be used for CD276 Molecule detection in Enzyme-Linked Immunosorbent Assay, Flow Cytometry and Immunoprecipitation. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen MCF7 cells
Epitope The epitope is in the B7-H3 extracellular domain.
Isotype IgG2a


Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.


Application ELISA; FC; IP


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target CD276
Alternative Name CD276 Molecule; Costimulatory Molecule; CD276 Antigen; B7 Homolog 3; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2
Gene ID 80381
UniProt Q5ZPR3
Research Area Immunology; Cancer Research
Related Disease Tumor
For research use only, not directly for clinical use.
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