| Description | This product is a human monoclonal antibody that reacts with CD33. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; DB; IF; IP; Neut; FuncS; ELISA; FC; ICC |
| Application Notes | The antibody is recommended for detection of CD33 by IF, IP, Neut, FuncS, ELISA, FC, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD33 |
| Alternative Name | CD33; CD33 molecule; CD33 antigen (gp67); myeloid cell surface antigen CD33; FLJ00391; p67; sialic acid binding Ig like lectin 3; SIGLEC 3; SIGLEC3; gp67; sialic acid binding Ig-lik |
| Gene ID | 945 |
| UniProt | P20138 |
| Related pathway | Hematopoietic Stem Cell Differentiation Pathways; Lineage-specific Markers; Innate Immune System |
| Research Area | Immunology; Stem Cell |
Figure 1 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) Fab Fragment in DB
Dot Blot analysis of V3S-0622-YC4319 Fab fragment was performed by coating with human CD33 protein (His tag).
V3S-0622-YC4319 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-His tag as a secondary antibody (1: 2000)
Figure 1 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) Fab Fragment in DB
Figure 2 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) Fab Fragment in WB
Western blot analysis of V3S-0622-YC4319 Fab fragment was performed by loading human CD33 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC4319 and HRP goat anti-His tag as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.5 μg)
Lane 2: Reduced antigen (0.8 μg)
Lane 3: Reduced antigen (1.0 μg)
Figure 2 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) Fab Fragment in WB
Figure 1 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in SEC-HPLC
The purity of V3S-0622-YC4319 was greater than 90% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV 280 nm
Injection: 25 µl
Figure 1 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in SEC-HPLC
Figure 2 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in WB
Western blot analysis of V3S-0622-YC4319 was performed by loading Recombinant Human CD33 Protein onto a 12% Tris-HCl polyacrylamide gel. The secondary antibody: Goat Anti-Human IgG-HRP.
Lane 1: Non-Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (0.5 μg)
Figure 2 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in WB
Figure 3 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in ELISA
ELISA analysis of V3S-0622-YC4319 was performed by coating with Recombinant Human CD33 Protein. Then blocked with BSA, and incubated with Anti-Human CD33 Antibody (V3S-0622-YC4319). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in ELISA
