Anti-CD33 Neutralizing Antibody (V3S-0622-YC4319) (CAT#: V3S-0622-YC4319)

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  • fig1
    Figure 1 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) Fab Fragment in DB
  • fig1
    Figure 2 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) Fab Fragment in WB
  • fig1
    Figure 1 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in SEC-HPLC
  • fig1
    Figure 2 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in WB
  • fig1
    Figure 3 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in ELISA

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with CD33. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; DB; IF; IP; Neut; FuncS; ELISA; FC; ICC
Application Notes The antibody is recommended for detection of CD33 by IF, IP, Neut, FuncS, ELISA, FC, ICC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CD33
Alternative Name CD33; CD33 molecule; CD33 antigen (gp67); myeloid cell surface antigen CD33; FLJ00391; p67; sialic acid binding Ig like lectin 3; SIGLEC 3; SIGLEC3; gp67; sialic acid binding Ig-lik
Gene ID 945
UniProt P20138
Related pathway Hematopoietic Stem Cell Differentiation Pathways; Lineage-specific Markers; Innate Immune System
Research Area Immunology; Stem Cell

Tested Data

DB

Figure 1 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) Fab Fragment in DB

Dot Blot analysis of V3S-0622-YC4319 Fab fragment was performed by coating with human CD33 protein (His tag).

V3S-0622-YC4319 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-His tag as a secondary antibody (1: 2000)

Figure 1 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) Fab Fragment in DB

WB

Figure 2 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) Fab Fragment in WB

Western blot analysis of V3S-0622-YC4319 Fab fragment was performed by loading human CD33 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC4319 and HRP goat anti-His tag as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.5 μg)
Lane 2: Reduced antigen (0.8 μg)
Lane 3: Reduced antigen (1.0 μg)

Figure 2 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) Fab Fragment in WB

HPLC

Figure 1 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in SEC-HPLC

The purity of V3S-0622-YC4319 was greater than 90% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV 280 nm
Injection: 25 µl

Figure 1 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in SEC-HPLC

WB

Figure 2 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in WB

Western blot analysis of V3S-0622-YC4319 was performed by loading Recombinant Human CD33 Protein onto a 12% Tris-HCl polyacrylamide gel. The secondary antibody: Goat Anti-Human IgG-HRP.
Lane 1: Non-Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (0.5 μg)

Figure 2 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in WB

ELISA

Figure 3 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in ELISA

ELISA analysis of V3S-0622-YC4319 was performed by coating with Recombinant Human CD33 Protein. Then blocked with BSA, and incubated with Anti-Human CD33 Antibody (V3S-0622-YC4319). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 3 Anti-CD33 Monoclonal Antibody (V3S-0622-YC4319) in ELISA

For research use only, not directly for clinical use.
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